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E-MTAB-6329

ABSTRACT: A nuclear-localized red fluorescence knock-in reporter allele for mouse pancreatic beta-cells

PROVIDER: E-MTAB-6329 | ExpressionAtlas | 2018-09-17

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-MTAB-6329-analysis-methods.tsv	Tabular
	E-MTAB-6329-analytics.tsv	Tabular
	E-MTAB-6329-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-6329-configuration.xml	Xml
	E-MTAB-6329-g1_g2-mvaPlot.png	Other

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A nuclear-localized red fluorescence knock-in reporter allele for mouse pancreatic beta-cells

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A nuclear-localized red fluorescence knock-in reporter allele for mouse pancreatic beta-cells

Project description:In this study, we generated a novel nuclear-localized red fluorescence knock-in reporter allele (Ins2.Apple) for mouse pancreatic beta-cells. Beta-cells were isolated by FACS from 60-day-old mice, segregated by sex, and RNA-sequencing was performed to assess sex-specific differences in beta-cell gene expression profiles. We also isolated beta-cells (by FACS) from MIP-GFP mice at 60 days of age. RNA-sequencing was performed, and was compared to that of Ins2.Apple beta cells, to assess gene expression changes brought on by the presence of the MIP-GFP transgene.

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