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E-MTAB-6336

ABSTRACT: Transcriptome changes in Slc7a5-null mouse embryos

PROVIDER: E-MTAB-6336 | ExpressionAtlas | 2019-01-16

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-MTAB-6336-analysis-methods.tsv	Tabular
	E-MTAB-6336-analytics.tsv	Tabular
	E-MTAB-6336-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-6336-configuration.xml	Xml
	E-MTAB-6336-g2_g1-mvaPlot.png	Other

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Transcriptome changes in Slc7a5-null mouse embryos

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Project description:The effects of loss of the large neutral amino acid transporter Slc7a5 (aka Lat1) on mouse embryonic development were investigated. Slc7a5 fl/fl mice harbouring two copies of the Slc7a5 targeted allele (exon 1 of Slc7a5 flanked with two loxP sites), were crossed with a mouse line ubiquitously expressing cre recombinase under the Bal1 promoter (Bal1-cre) to obtain a global Slc7a5 knockout mouse (Poncet et al. 2014 PLoS One 9: e89547). Heterozygous Slc7a5+/- C57Bl/6 mice were viable and fertile and were bred free of Bal1-cre in subsequent generations. Slc7a5 -/- embryos were obtained by inter-crossing heterozygotes and a phenotype was apparent by E9.5. To identify the first cellular processes affected by Slc7a5 loss RNAseq was carried out to compare transcriptomes of null and wildtype E8.5 embryos.

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