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E-MTAB-7013

ABSTRACT: RNA-seq of Drosophila melanogaster testis with or without tbrd-1 knock out or tplus3a/b knock out

PROVIDER: E-MTAB-7013 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-MTAB-7013-analysis-methods.tsv	Tabular
	E-MTAB-7013-analytics.tsv	Tabular
	E-MTAB-7013-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-7013-configuration.xml	Xml
	E-MTAB-7013-g3_g1-mvaPlot.png	Other

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RNA-seq of Drosophila melanogaster testis with or without tbrd-1 knock out or tplus3a/b knock out

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RNA-seq of Drosophila melanogaster testis with or without tbrd-1 knock out or tplus3a/b knock out

Project description:Many protein complexes are involved in gene regulation during Drosophila spermatogenesis. tplus3a and tplus3b code for Plus3 domain proteins and are enriched in spermatocytes. Male flies ∆tplus3a/b carrying deletions of both genes in trans to deficiency Df(2L)BSC151 show severely reduced fertility. tbrd-1 is known to regulate hundreds of genes during spermatogenesis in cooperation with tTAFs. To gain more insight into the regulatory mechanisms during spermatogenesis we used RNA from testes of ∆tplus3a/b/Df(2L)BSC151 and tbrd-11 mutant flies for RNAseq in comparison to w1118 wild-typic control. RNA was isolated with Trizol from 200 Testes dissected from w1118, homozygous tbrd-11 mutants or ∆tplus3a/b/Df(2L)BSC151. After DNAse I digestion RNA was purified and RNAseq was performed in three replicates. Genotype Description: w1118: wild-typic control tbrd-11: homozygous tbrd-11 mutants (knock-out through P{EPgy2}tbrd-11, as described in Leser et al., 2012) ∆tplus3a/b/Df(2L)BSC151: CRISPR/Cas9 deletion mutants, representing knock-out of both tplus3 and tplus3b. Male flies y1cho2v1;∆tplus3a/b/CyO were crossed with virgin females w1118;Df(2L)BSC151/CyO. Male offspring with w1118;∆tplus3a/b/Df(2L)BSC151 were used for RNAseq.

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