Project description:To identify targets of tankyrase 1 and 2 we established a double knock out HEK293T cell line.
We compared the proteins in WT versus the double knock out applying a relative quantitation method using TMT tags and off-line fractionation of the proteome.
Project description:We modified the CiGEnC cell line by CRISPR Cas9 genome edition to suppress both classI and classII HLA molecules production by CIITA and B2M knock out. After 7 days of differentiation at 37°C, we extracted RNA from the cell line and performed bulk RNA sequencing to provide a basal profile of the whole transcriptom of the cell line
Project description:Purpose:Next-generation sequencing has revolutionized sytems-level celluar pathway analysis. The goals of this study are to compare the U87 cell xenograft GBM mice (U87 cell line) to TWIST1 knock out U87 cell xenograft GBM mice (TWIST1 knock out U87 cell line) using their transcriptomes
Project description:This analysis was performed among 786-o renal cell carcinoma cell line, its sunitinib resistant cell, and sunitinib resistant Hif2a knock out cell.
Project description:We have analyzed the transcript expression levels in Dicer heterozygous and Dicer knock-out embryonic stem (ES) cells in order to identify which transcripts are regulated by RNAi pathway in mouse ES cells. Keywords: Cell type comparison of cell line with or without knock-out