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E-MTAB-7315

ABSTRACT: RNA-seq analysis of colonic intestinal epithelial cells (IECs) isolated from naive wild-type C57BL/6 mice, DSS-treated wild-type C57BL/6 mice, Il10-/- C57BL/6 mice and Il10-/- C57BL/6 mice with IEC-specific Rabgef1 deletion

PROVIDER: E-MTAB-7315 | ExpressionAtlas | 2019-01-16

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-7315-analysis-methods.tsv	Tabular
	E-MTAB-7315-analytics.tsv	Tabular
	E-MTAB-7315-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-7315-configuration.xml	Xml
	E-MTAB-7315-g4_g1-mvaPlot.png	Other

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RNA-seq analysis of colonic intestinal epithelial cells (IECs) isolated from naive wild-type C57BL/6 mice, DSS-treated wild-type C57BL/6 mice, Il10-/- C57BL/6 mice and Il10-/- C57BL/6 mice with IEC-specific Rabgef1 deletion

Project description:The aim of this study is to compare the transcriptomic profile of colonic intestinal epithelial cells (IECs) at steady-state, and in several models of colitis: in naive wild-type C57BL/6 mice, in wild-type C57BL/6 mice treated for 7 days with 3% dextran sodium sulfate (DSS) in drinking water, in Il10-/- C57BL/6 mice and Il10-/- C57BL/6 mice with IEC-specific Rabgef1 deletion. Colonic IECs were isolated and FACS-sorted, then subjected tu bulk RNA-seq.

2019-09-30 | E-MTAB-7315 | biostudies-arrayexpress

Project description:RNA-seq analysis of colonic intestinal epithelial cells (IECs) isolated from naive wild-type C57BL/6 mice, DSS-treated wild-type C57BL/6 mice, Il10-/- C57BL/6 mice and Il10-/- C57BL/6 mice with IEC-specific Rabgef1 deletion

| PRJEB29259 | ENA

E-GEOD-49804

Project description:Expression data from dexamethasone treated mouse embryonic neural progenitor/stem cells isolated from wild type C57Bl/6 or caveolin-1 knockout mice

2015-07-27 | E-GEOD-49804 | ExpressionAtlas

Immunoregulatory actions of T cell PPAR g at the colonic mucosa

Project description:BACKGROUND: Peroxisome proliferator-activated receptor g (PPAR g) is a nuclear receptor whose activation has been shown to modulate macrophage and epithelial cell-mediated inflammation. The objective of this study was to use a systems approach for investigating the mechanism by which the deletion of PPAR g in T cells modulates the severity of dextran-sodium sulfate (DSS)-induced colitis, immune cell distribution and global gene expression. METHODS: Wild-type (WT) or PPAR g flfl; CD4 Cre+ (CD4cre) mice in a C57BL/6 background were challenged with 2.5% DSS in their drinking water for 0, 2, or 7 days. Mice were scored on disease severity both clinically and histopathologically. Flow cytometry was used to assess lymphocyte and macrophage populations in the blood, spleen, and mesenteric lymph nodes (MLN). Global gene expression in colonic mucosa was profiled using Affymetrix microarrays. RESULTS: Both disease severity and inflammation-related body weight loss were accelerated by the deficiency of PPAR g in T cells. Examination of colon histopathology revealed significantly greater epithelial erosion, leukocyte infiltration, and mucosal thickening in the CD4cre mice on day 7. CD4cre mice had more CD8+ T cells than wt mice and fewer CD4+FoxP3+ regulatory T cells (Treg) and IL10+CD4+ T cells in blood and MLN, respectively. Transcriptomic profiling revealed around 3000 genes being transcriptionally altered as a result of DSS challenge in CD4cre mice. These included up-regulated adhesion molecules on day 7 and proinflammatory cytokines interleukin-6 (IL-6) and IL-1b, and suppressor of cytokine signaling 3 (SOCS-3) mRNA expression. CONCLUSIONS: These findings suggest that T cell PPAR g down-regulates inflammation during DSS colitis by inhibiting colonic expression of inflammatory mediators and increasing MLN Treg. Colonic mucosa from wt and CD4cre mice were sampled at 0 (no DSS), 2, and 7 days of DSS-induced experimental colitis

2010-07-08 | E-GEOD-20523 | biostudies-arrayexpress

E-MTAB-6547

Project description:RNAseq analysis of colon and ileum tissue from naive wild type, MR1-/- and IL-17A-/- mice

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Genome-wide mRNA expression profiling for Wild Type and Itch-/- colonic mucosa Transcriptomes by RNA sequencing

Project description:To test the hypothesis that defects in the termination of inflammatory signaling led to colon inflammation, we isolated RNA from the colonic mucosa of Itch-/- mice and from the colonic mucosa of wild-type mice treated with DSS and performed genome-wide mRNA expression profiling by RNA sequencing.

2016-06-20 | GSE81066 | GEO

E-MTAB-9148

Project description:Transcriptomic analysis of the distal colon of wild type C57BL/6N mice and mice harboring a mutation (S839I) in Gucy2c

2021-03-22 | E-MTAB-9148 | ExpressionAtlas

E-GEOD-59672

Project description:Expression data from DOX(doxorubicin) -treated wild type or CHIP knockout C57BL/6J mice at day 5

2015-07-29 | E-GEOD-59672 | ExpressionAtlas

E-MTAB-4924

Project description:Lipidomic and Metabolic Changes in the P4-type ATPase ATP10D deficient C57BL/6J Wild Type Mice upon Rescue of ATP10D Function

2016-07-05 | E-MTAB-4924 | ExpressionAtlas

p38a-dependent gene expression in the colonic mucosa

Project description:Gene expression in the colonic mucosa of wild-type and p38a-knockout intestinal epithelial cells (IECs) were compared. C57BL/6 wild-type mice, and intestinal epithelial cell-specific p38a-knockout mice on a C57BL/6 background were used for isolation of colonic mucosa

2014-07-01 | GSE55356 | GEO

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