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E-MTAB-7374

ABSTRACT: Salicylic acid-induced gene expression in wild-type Col-0 and mutant upl3-4 Arabidopsis thaliana plants.

PROVIDER: E-MTAB-7374 | ExpressionAtlas | 2019-01-16

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-7374-analysis-methods.tsv	Tabular
	E-MTAB-7374-analytics.tsv	Tabular
	E-MTAB-7374-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-7374-configuration.xml	Xml
	E-MTAB-7374-g2_g1-mvaPlot.png	Other

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Salicylic acid-induced gene expression in wild-type Col-0 and mutant upl3-4 Arabidopsis thaliana plants.

Project description:Salicylic acid-induced gene expression in wild-type Col-0 and mutant upl3-4 Arabidopsis thaliana plants.

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Salicylic acid-induced gene expression in wild-type Col-0 and mutant upl3-4 Arabidopsis thaliana plants.

Project description:Activation of plant immunity is associated with dramatic transcriptome reprogramming to prioritise immune responses over normal cellular functions. Changes in gene expression are coordinated by the immune hormone salicylic acid (SA). Here we investigated the involvement of the HECT-type ligase Ubiquitin Protein Ligase 3 (UPL3) in SA-induced transcriptional reprogramming. We show that UPL3 acts as an amplifier of SA-induced changes in gene expression. Four-week old Arabidopsis thaliana plants of wild-type Col-0 and mutant upl3-4 genotypes were germinated on soil in 100% relative humidity. After 12 days plants were transplanted to larger pots (six plants per pot) and grown for an additional 3 weeks before experimental treatment. Plants were continuously grown in an environmental chamber with 16/8 hour day/night light regime (120 mol m-2 s-1 light intensity), 21/18 degrees celcius day/night cycle and 65% relative humidity. Plants were then sprayed with water or 0.5 mM SA until all leaves were thoroughly covered with fine droplets. After 24 hours leaf tissue was harvested from 6 plants per treatment and pooled together into a single biological repeat. In total two or three independent biological repeats were collected. After harvesting leaf tissue was immediately frozen in liquid nitrogen until further analysis.

2018-11-09 | E-MTAB-7374 | biostudies-arrayexpress

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Project description:RNAseq analysis of Arabidopsis Col-0 wild-type and fbl17 mutant seedlings

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Salicylic acid-induced gene expression in wild-type Col-0 and mutant ube4 Arabidopsis thaliana plants

Project description:Salicylic acid-induced gene expression in wild-type Col-0 and mutant ube4 Arabidopsis thaliana plants

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Arabidopsis gene expression profile : Col-0 wild-type vs. pif3pif4pil5pil6 (pifQ) mutant

Project description:Transcriptional profiling of 60h-old Arabidopsis whole seedlings comparing control Col-0 wild-type plants with pifQ mutant plants The expression profile of dark-grown pifQ mutant shows similar pattern of Rc-grown Col-0 wild-type Keywords: Genetic modification

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1-aminocyclopropane-1-carboxylic acid (ACC)-induced gene expression in wild-type Col-0, mutant upl3 upl4, mutant ein3-1, and mutant upl3 upl4 ein3-1 Arabidopsis thaliana plants

Project description:1-aminocyclopropane-1-carboxylic acid (ACC)-induced gene expression in wild-type Col-0, mutant upl3 upl4, mutant ein3-1, and mutant upl3 upl4 ein3-1 Arabidopsis thaliana plants

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Project description:Comparison of low water potential (drought)-regulated gene expression in wild type (Col-0) and the hai1-2 (At5g59220) mutant

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1-aminocyclopropane-1-carboxylic acid (ACC)-induced gene expression in wild-type Col-0, mutant upl3 upl4, mutant ein3-1, and mutant upl3 upl4 ein3-1 Arabidopsis thaliana plants

Project description:The plant hormone ethylene is involved in plant developmental, stress and environmental signalling. The ethylene signalling pathway is modulated by the master transcription factor EIN3. Here we examined the involvement of proteasome-associated UPL3 and UPL4 ubiquitin ligases in ethylene-responsive gene expression. We reveal that in absence of functional UPL3 and UPL4, plants show constitutive and enhanced activation or repression of ethylene-responsive genes in an EIN3-dependent manner. Ten-day old Arabidopsis thaliana seedlings of wild-type Col-0, mutant upl3 upl4, mutant ein3-1, and mutant upl3 upl4 ein3-1 were grown on MS media in an environmental chamber with 16/8 hour day/night light regime (120 mol m-2 s-1 light intensity) and 22 degrees Celsius. Seedlings were then transferred to 6-well plates and floated on the surface of water or 50 uM 1-aminocyclopropane-1-carboxylic acid (ACC). After 3 hours seedlings were harvested and for each treatment ~50 seedlings were pooled together into a single biological repeat. In total three independent biological repeats were collected. After harvesting seedlings were briefly dried on tissue and immediately frozen in liquid nitrogen until further analysis.

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Salicylic acid-induced gene expression in wild-type Col-0 and mutant upl1, upl5, and npr1-1 Arabidopsis thaliana plants

Project description:Salicylic acid-induced gene expression in wild-type Col-0 and mutant upl1, upl5, and npr1-1 Arabidopsis thaliana plants

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