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E-MTAB-8520

ABSTRACT: Effect of heat stress on developing wheat grain

PROVIDER: E-MTAB-8520 | ExpressionAtlas | 2020-01-10

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-8520-analysis-methods.tsv	Tabular
	E-MTAB-8520-analytics.tsv	Tabular
	E-MTAB-8520-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-8520-configuration.xml	Xml
	E-MTAB-8520-g1_g2-mvaPlot.png	Other

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Effect of heat stress on developing wheat grain

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Effect of heat stress on developing wheat grain

Project description:Effect of heat stress on developing wheat grain

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Small RNA, transcriptome and degradome analysis of the transgenerational heat stress response network in durum wheat

Project description:Heat stress is a major limiting factor for grain yield and grain quality in wheat production. In crops, abiotic stresses have transgenerational effects and the mechanistic basis of stress memory is associated with epigenetic regulation. The current study presents the first systematic analysis of the transgenerational effects of post-anthesis heat stress in tetraploid wheat. Genotype-dependent response patterns to parental and progeny heat stress were found for the leaf physiological traits, harvest components, and grain quality traits measured. Parental heat stress had positive influence on the offspring under re-occurring stress for traits like chlorophyll content, grain weight, grain number and grain total starch content. Integrated sequencing analysis of the small RNAome, mRNA transcriptome, and mRNA degradome provided the first description of the molecular networks mediating heat stress adaption under transgenerational influence. The expression profile of 1771 microRNAs (733 being novel) and 66,559 genes was provided, with differentially expressed microRNAs and genes identified subject to the progeny treatment, parental treatment and tissue type factors. Gene Ontology and KEGG pathway annotation of stress responsive microRNAs-mRNA modules provided further information on their functional roles in biological processes like hormone homeostasis, signal transduction, and protein stabilization. Our results provide new sights on the molecular basis of transgenerational heat stress adaptation, which can be used for improving thermos-tolerance in breeding.

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Transcriptomic and physiological response of durum wheat grain to short period of heat stress at the early grain-filling stage

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Project description:Background and aims Climate warming has become an indisputable fact, and wheat is among the most heat-sensitive cereal crops. Heat stress during grain filling threatens global wheat production and food security. Here, we analyzed the physiological and proteomic changes by delayed sowing on the photosynthetic capacity of winter wheat leaves under heat stress. Our aim is to provide a new cultivation way for the heat stress resistance in wheat. Methods Through 2 years field experiment and an open warming simulation system, we compared the changes in wheat grain weight, yield, photosynthetic rate, and chlorophyll fluorescence parameters under heat stress at late grain–filling stage during normal sowing and delayed sowing. At the same time, based on the iTRAQ proteomics, we compared the changes of differentially expressed proteins (DEPs) during the two sowing periods under high temperature stress.

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Microarray analysis of heat and/or drought sensitivity of early grain development in wheat (Triticum aestivum L.)

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