E-MTAB-8520
Ontology highlight
ABSTRACT:
PROVIDER: E-MTAB-8520 | ExpressionAtlas | 2020-01-10
REPOSITORIES: ExpressionAtlas
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Project description:effect of post anthesis heat stress on transcriptome of developing wheat grain
2019-12-19 | E-MTAB-8520 | biostudies-arrayexpress
Project description:Effect of heat stress on developing wheat grain
| PRJEB35451 | ENA
Project description:Heat stress is a major limiting factor for grain yield and grain quality in wheat production. In crops, abiotic stresses have transgenerational effects and the mechanistic basis of stress memory is associated with epigenetic regulation. The current study presents the first systematic analysis of the transgenerational effects of post-anthesis heat stress in tetraploid wheat. Genotype-dependent response patterns to parental and progeny heat stress were found for the leaf physiological traits, harvest components, and grain quality traits measured. Parental heat stress had positive influence on the offspring under re-occurring stress for traits like chlorophyll content, grain weight, grain number and grain total starch content. Integrated sequencing analysis of the small RNAome, mRNA transcriptome, and mRNA degradome provided the first description of the molecular networks mediating heat stress adaption under transgenerational influence. The expression profile of 1771 microRNAs (733 being novel) and 66,559 genes was provided, with differentially expressed microRNAs and genes identified subject to the progeny treatment, parental treatment and tissue type factors. Gene Ontology and KEGG pathway annotation of stress responsive microRNAs-mRNA modules provided further information on their functional roles in biological processes like hormone homeostasis, signal transduction, and protein stabilization. Our results provide new sights on the molecular basis of transgenerational heat stress adaptation, which can be used for improving thermos-tolerance in breeding.
2021-05-24 | GSE171303 | GEO
Project description:Heat stress is one of the major abiotic stress factor that affects wheat yield. Especially, heat stress during grain filling affects grain yield besides reduced grain quality. So, in our present study, three genotypes with varied levels of tolerance to heat stress were chosen. They were subjected to heat stress at two stages for three days viz., early (11-14days-post-anthesis) and late (27-30dpa) grain filling independently under controlled conditions. At 14 and 30dpa, the spikes were harvested from control and stress conditions from all the three genotypes, grains were isolated and pulverized. Hence pulverized tissues are used for RNA extraction and further for transcriptome sequencing using HiSeq 4000. Data were analyzed to identify the genes involved in imparting heat stress tolerance.
2019-12-16 | E-MTAB-6140 | biostudies-arrayexpress
Project description:Wheat is one of the most significant crops in terms of human consumption in the world. In a climate change scenario, extreme weather event such as heatwaves will be more frequent especially during the grain-filling (GF) stage and could affect grain weight and quality of crops. Molecular mechanisms underlying the response to short heat stress (HS) have been widely reported for the hexaploid wheat (Triticum aestivum) but the regulatory heat stress mechanisms in tetraploid durum wheat (Triticum turgidum ssp. durum) remain partially understood. In this work, we performed a transcriptomic analysis of durum wheat grains to HS during early GF to identify key HS response genes and their predicted regulatory networks under glasshouse conditions.
2021-10-26 | GSE186472 | GEO
Project description:Background and aims Climate warming has become an indisputable fact, and wheat is among the most heat-sensitive cereal crops. Heat stress during grain filling threatens global wheat production and food security. Here, we analyzed the physiological and proteomic changes by delayed sowing on the photosynthetic capacity of winter wheat leaves under heat stress. Our aim is to provide a new cultivation way for the heat stress resistance in wheat. Methods Through 2 years field experiment and an open warming simulation system, we compared the changes in wheat grain weight, yield, photosynthetic rate, and chlorophyll fluorescence parameters under heat stress at late grain–filling stage during normal sowing and delayed sowing. At the same time, based on the iTRAQ proteomics, we compared the changes of differentially expressed proteins (DEPs) during the two sowing periods under high temperature stress.
2022-02-26 | PXD031871 | Pride
Project description:Based on EST-based in silico gene expression analysis a 15k oligonucleotid microarray has been developped in order to monitor environmental stress-dependent gene expression changes in the wheat caryopsis. Using this array, the effect of water withdrawal, with and and without additional heat stress, during the first five days of grain development (0-5 DAA) has been investigated on two wheat cultivars differing in their drought sensitivity. The combined effect of heat and drought (DH) on gene expression was much significant (8-10% of the investigated genes changed >2-fold) in contrast to drought alone (1.5%). Drought and heat stress resulted in the co-ordinated change of the expression of storage proteins, some enzymes involved in sugar/starch metabolism, cell division-related and histone proteins, certain transcription factors, heat shock proteins, proteases and aquaporins. The potential link between the observed gene expression changes and the parallel histological observations indicating the accelerated development of the stressed grains is discussed.
2009-09-24 | GSE18205 | GEO
Project description:A heat and drought tolerant rice cultivar (N22) was grown in the field under control and drought conditions during the dry season in 2013. Drought was applied during early grain filling and resulted in simultaneous heat stress, leading to reduced grain yield and quality. Total RNA was extracted from developing seeds under stress and control (fully flooded) conditions and RNA-seq analysis was performed. These samples are a part of a bigger experiment analysing the responses of three contrasting rice cultivars (N22, Dular, Anjali) to combined heat and drought stress including different organs (developing seeds, flag leaves, flowering spikelets) and developmental stages (early grain filling, flowering) at the transcriptomic level.
2020-11-04 | GSE153030 | GEO
Project description:Based on EST-based in silico gene expression analysis a 15k oligonucleotid microarray has been developped in order to monitor environmental stress-dependent gene expression changes in the wheat caryopsis. Using this array, the effect of water withdrawal, with and and without additional heat stress, during the first five days of grain development (0-5 DAA) has been investigated on two wheat cultivars differing in their drought sensitivity. The combined effect of heat and drought (DH) on gene expression was much significant (8-10% of the investigated genes changed >2-fold) in contrast to drought alone (1.5%). Drought and heat stress resulted in the co-ordinated change of the expression of storage proteins, some enzymes involved in sugar/starch metabolism, cell division-related and histone proteins, certain transcription factors, heat shock proteins, proteases and aquaporins. The potential link between the observed gene expression changes and the parallel histological observations indicating the accelerated development of the stressed grains is discussed. 8 samples with 2 biological replicate using dye swap
2010-05-22 | E-GEOD-18205 | biostudies-arrayexpress
Project description:Wheat yield is correlated significantly with grain size which is established during morphological stage. In present study, morphological stage of developing wheat grain were analyzed by RNA-seq.This research will help us to understand the mechnism underlying of grain development. This is the first study on gene expression profiling of morphological stage of developing wheat grain and the results may aid the identification of pathways and genes associated with seed development in wheat.
2014-01-15 | E-MTAB-4260 | biostudies-arrayexpress