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E-MTAB-8697

ABSTRACT: RNA-Seq analysis of Medicago truncatula hairy root lines treated with methyl jasmonate

PROVIDER: E-MTAB-8697 | ExpressionAtlas | 2020-07-20

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-8697-analysis-methods.tsv	Tabular
	E-MTAB-8697-analytics.tsv	Tabular
	E-MTAB-8697-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-8697-configuration.xml	Xml
	E-MTAB-8697-g3_g1-mvaPlot.png	Other

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RNA-Seq analysis of Medicago truncatula hairy root lines treated with methyl jasmonate

Project description:RNA-Seq analysis of Medicago truncatula hairy root lines treated with methyl jasmonate

| PRJEB36289 | ENA

RNA-Seq analysis of Medicago truncatula hairy root lines treated with methyl jasmonate

Project description:In hairy roots of the model legume Medicago truncatula the saponin production can be elicited by methyl jasmonate treatment. To identify genes potentially involved in saponin biosynthesis or its regulation we carried out transcript profiling by RNA-Seq of M. truncatula hairy roots treated with 100 μM of methyl jasmonate (dissolved in ethanol) for two or 24 hours. As control, M. truncatula hairy roots treated with an equivalent amount of ethanol were profiled.

2020-01-27 | E-MTAB-8697 | biostudies-arrayexpress

The effect of loss of Makibishi1-(MKB1) function on the transcriptome of Medicago truncatula hairy roots

Project description:RNA-Seq analysis on Medicago truncatula hairy roots, either control lines or lines silenced for the Makbishi1 (MKB1) gene, grown in either mock conditions or elicited with the plant stress hormone methyl jasmonate for 4 hours

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Project description:Transcription profiling by high throughput sequencing of Medicago truncatula hairy root lines overexpressing TSAR1 or TSAR2

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Project description:Hairy root lines over-expressing MtPAR using a 35S promoter compared with hairy lines over-expressing GUS gene. Hairy roots were generated in vitro using leaf explants from Medicago A17. The goal of this experiment is to prove that ectopic expression expression of MtPAR is sufficient to induce tannin biosynthesis

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Rhizogenic Agrobacterium protein RolB interacts with the TOPLESS and NINJA repressor proteins to reprogram plant jasmonate signaling (GUS overexpression)

Project description:RNA sequencing was performed on tomato hairy root lines transformed with a GUS overexpression construct, 24 h after mock and jasmonate (JA) treatment. This set-up allowed us to map the gene targets of JA in tomato hairy roots after 24h.

2022-07-08 | E-MTAB-11876 | biostudies-arrayexpress

E-MTAB-6991

Project description:RNA-seq analysis of PROX1 overexpression and knockdown in glioblastoma cell lines

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Transcription profiling of Medicago truncatula TTG1 over-expressing hairy root

Project description:Transcriptomics analysis of MtTTG1 over-expressing hairy root in the background of Medicago truncatula genotype Jemalong A17.

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SWATH-MS Proteomics Analysis of Persicaria minor herb upon Methyl Jasmonate elicitation

Project description:Stresses from either biotic or abiotic origins can have significant impact towards plant physiology and molecular regulation. Jasmonate acid (JA) and its derivative, methyl JA (MeJA) are hormonal cues released by plants which signal defensive response to curb the damage from such stresses. In an attempt to study the defensive response, a tropical herbal plant, Persicaria minor (P. minor) which is known for its pungent smell as well as various bioactivities including antimicrobial and anti-cancer, has been treated with MeJA to invoke the stress signaling. Such elicitation has been performed in various plants such as Arabidopsis, rice and hairy root cultures of certain herbs, yet how MeJA directly influenced the proteome of a herbal species particularly P. minor has not been previously elucidated. In this study, P. minor plants was exogenously treated with MeJA and its proteome was investigated using a new proteomics approach called SWATH-MS.

2018-06-25 | PXD005749 | Pride

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