E-MTAB-8840
Ontology highlight
ABSTRACT:
PROVIDER: E-MTAB-8840 | ExpressionAtlas | 2020-07-20
REPOSITORIES: ExpressionAtlas
Similar Datasets
Project description:Tissue-autonomous regulation of adipose expansion by core clock protein REVERBα
| PRJEB37041 | ENA
Project description:The circadian clock coordinates energy metabolism, with REVERBα considered a dominant regulator of lipid metabolism. REVERBα is a core clock protein, which demonstrates robust rhythmic expression across metabolic tissues. Here, we explore its role in white adipose metabolism, and propose that REVERBα plays a key part in the response of adipose to obesity. Mice globally lacking Reverbα (Reverbα-/-) display adiposity, increased susceptibility to diet-induced obesity, and up-regulation of the lipogenic proteome in white adipose depots. When Reverbα is deleted selectively in adipose (ReverbαFlox2-6AdipoCre), however, we observe only modest phenotypic and transcriptomic changes in the basal state, with altered regulation of clock and extracellular matrix (ECM) pathways. Thus the adipose-autonomous actions of REVERBα are shaped by context. On high-fat diet challenge, ReverbαFlox2-6AdipoCre mice accumulate more adipose mass than littermate controls, and are spared obesity-related inflammation. Gene expression analysis reveals pathways of lipid handling to be up-regulated. These findings therefore highlight a critical role for REVERBα in the regulation of adipose tissue expansion. Further, when transcriptomic data is integrated with the REVERBα cistrome, we detect strong associations of REVERBα binding sites, not only with the clock and ECM targets de-repressed under normal conditions, but with the broad range of metabolic genes unmasked as REVERBα targets by obesity. Thus our data favour a new interpretation of REVERBα function, not as conferring rhythmicity to lipogenesis, but as buffering against lipogenic cues asynchronous to the normal temporal routine.
2021-07-31 | E-MTAB-8840 | biostudies-arrayexpress
Project description:Mouse interscapular brown, subscapular white and inguinal white adipose tissue
2023-08-04 | E-MTAB-9192 | ExpressionAtlas
Project description:Mouse adipose tissue under hypercaloric and isocaloric high-fat diet
2019-08-30 | E-MTAB-7755 | ExpressionAtlas
Project description:Thyroid deficiency before birth modifies adipose transcriptome to promote overgrowth of white adipose tissue and impair thermogenic capacity
2023-06-16 | E-MTAB-8396 | ExpressionAtlas
Project description:RNA-seq of gonadal adipose tissue from male and female Trim28-WT and Trim28 adipose specific KO mice
2020-12-09 | E-MTAB-9809 | ExpressionAtlas
Project description:RNA-seq of age-associated transcriptome changes in brown adipose tissue
2019-01-16 | E-MTAB-7445 | ExpressionAtlas
Project description:Circadian and metabolic physiology are intricately intertwined, as illustrated by Rev-erb , a transcription factor (TF) that functions both as a core repressive component of the cell autonomous clock and as a regulator of metabolic genes. Here we show that Rev-erb modulates the clock and metabolism by different genomic mechanisms. Clock control requires Rev-erb to bind directly to the genome at its cognate sites, where it competes with activating ROR TFs. By contrast, Rev-erb regulates metabolic genes primarily by recruiting the HDAC3 corepressor to sites to which it is tethered by cell type-specific transcription factors. Thus, direct competition between Rev-erb and ROR TFs provides a universal mechanism for self-sustained control of molecular clock across all tissues, whereas Rev-erb utilizes lineage-determining factors to convey a tissue-specific epigenomic rhythm that regulates metabolism tailored to the specific need of that tissue. Biological replicates were uploaded in separated files and indicated in the file names.
2015-06-16 | E-GEOD-67962 | biostudies-arrayexpress
Project description:RNA-seq of male and female mouse brown adipose tissue (BAT) transcriptome
2020-07-20 | E-MTAB-8717 | ExpressionAtlas
Project description:Bulk mRNA-seq of brown adipose tissue derived from SLC25A44 knockdown mouse
2019-09-09 | E-MTAB-7987 | ExpressionAtlas