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E-MTAB-8903

ABSTRACT: RNA-Seq of BEAS-2B cells treated with low doses of Ni and NiO nanoparticles as well as NiCl2 for 6 weeks

PROVIDER: E-MTAB-8903 | ExpressionAtlas | 2025-01-16

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-8903-analysis-methods.tsv	Tabular
	E-MTAB-8903-analytics.tsv	Tabular
	E-MTAB-8903-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-8903-configuration.xml	Xml
	E-MTAB-8903-g4_g1-mvaPlot.png	Other

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RNA-Seq of BEAS-2B cells treated with low doses of Ni and NiO nanoparticles as well as NiCl2 for 6 weeks

Project description:Human bronchial lung cells (BEAS-2B) were treated for 6 weeks with low doses (0.5 µg/mL) of Ni and NiO nanoparticles and NiCl2. At the end of the exposure control and treated samples were submitted for RNA-Seq analysis (Hiseq2500). The overall goal of this experiment was to gain an in-depth understanding of the transcriptomic changes induced by low-dose, long-term exposure of human lung cells to Ni and NiO nanoparticles as well as to NiCl2 and to generate hypotheses related to their mechanisms of toxicity.

2020-04-19 | E-MTAB-8903 | biostudies-arrayexpress

RNA-Seq of BEAS-2B cells treated with low doses of Ag nanoparticles for 6 weeks

Project description:Human bronchial lung cells (BEAS-2B) were treated for 6 weeks with low doses (1 µg/mL) of Ag nanoparticles (10 nm citrate coated). At the end of the exposure control and treated samples were submitted for RNA-Seq analysis (Hiseq2500). The overall goal of this experiment was to gain an in-depth understanding of the transcriptomic changes induced by low-dose, long-term exposure of human lung cells to Ag nanoparticles and to generate hypotheses related to their mechanisms of toxicity.

2018-04-30 | E-MTAB-6321 | biostudies-arrayexpress

DNA methylation profiling of BEAS-2B cells exposed to low doses of Ag nanoparticles for 6 weeks

Project description:Human bronchial lung cells (BEAS-2B) were treated for 6 weeks with low doses (1 µg/mL) of Ag nanoparticles (10 nm citrate-coated). At the end of the exposure control and treated samples were submitted for DNA methylation analysis (Illumina 450k). The overall goal of this experiment was to evaluate potential epigenetic changes associated with low-dose, long-term exposure to Ag nanoparticles.

2018-04-30 | E-MTAB-6331 | biostudies-arrayexpress

RNA-Seq of BEAS-2B cells treated with low doses of Ag nanoparticles for 6 weeks

Project description:RNA-Seq of BEAS-2B cells treated with low doses of Ag nanoparticles for 6 weeks

| PRJEB24011 | ENA

RNA-Seq of BEAS-2B cells treated with low doses of Ni and NiO nanoparticles as well as NiCl2 for 6 weeks

Project description:RNA-Seq of BEAS-2B cells treated with low doses of Ni and NiO nanoparticles as well as NiCl2 for 6 weeks

| PRJEB37703 | ENA

Effects of chronic exposure of human bronchial epithelial cells to low doses of cigarette smoke condensate

Project description:BEAS-2B cells have been treated with low doses (20µg/ml) of CSC for 4 months. As negative control BEAS-2B cells were treated with DMSO (the CSC solvent). Non-treated cells were cultivated in parallel. Keywords: time course, stress response

2009-08-01 | GSE14383 | GEO

Transcription profiling of human bronchial epithelial cells to low doses of cigarette smoke condensate - chronic exposure

Project description:BEAS-2B cells have been treated with low doses (20 ug/ml) of CSC for 4 months. As negative control BEAS-2B cells were treated with DMSO (the CSC solvent). Non-treated cells were cultivated in parallel. Experiment Overall Design: After each month total RNA was extracted from three replicates of CSC, DMSO and non-treated BEAS-2B cells and hybridized to Affymetrix GeneChips.

2009-08-09 | E-GEOD-14383 | biostudies-arrayexpress

Expression data from human lung epithelial cells after amorphous silica nanoparticle exposure for 40 passages

Project description:Amorphous silica nanoparticles induce malignant transformation and tumorigenesis of human lung epithelial cells. We used microarrays to detail the global programme of gene expression underlying the cellular malignant transformation induced by amorphous silica nanoparticles and identified distinct classes of up-regulated and down-regulated genes during this process. The human lung epithelial cells, Beas-2B were continuously exposed to 5 Î¼g/mL amorphous silica nanoparticles for 40 passages, and named as BeasSiNPs-P40 (shortly as P40-5 during the further microarray detection). Meanwhile, the passage-matched control Beas-2B cells, named as Beas-P40 (shortly as NC during the further microarray detection).

2016-06-01 | E-GEOD-82062 | biostudies-arrayexpress

high-throughput sequencing of circRNAs in T-BEAS-2B cells and C-BEAS-2B cells

Project description:We report the differential expression of circRNAs between T-BEAS-2B cells (cadmium-transformed BEAS-2B cells) and C-BEAS-2B cells (passage-matched control BEAS-2B cells) by high-throughput sequencing. T-BEAS-2B cells are BEAS-2B cells transformed by cadmium at 2.0 μM for twenty weeks, and C-BEAS-2B cells are their passage-matched control. RNAs were sequenced on Illumina HiSeq Xten platform in triplicates, and expressions of circRNAs were calculated by TPM (transcripts per kilobase of exon model per million mapped reads). Clean data per sample exceeds 10 GB. We find 235 significantly up-regulated circRNAs and 271 significantly down-regulated circRNAs in T-BEAS-2B cells relative to C-BEAS-2B cells. Our work provides clues and evidence for exploring the mechanism of circRNAs in cadmium carcinogenesis.

2024-01-26 | GSE165549 | GEO

Comparison of gene expression profiles in chromate transformed BEAS-2B cells

Project description:We established chromate transformed cell lines by chronic exposure of normal human bronchial epithelial BEAS-2B cells to low doses of hexavalent chromium followed by anchorage-independent growth. The gene expression profiles were analyzed in the established cell lines. The gene expression profiles from six chromate transformed cell lines were remarkably similar to each other yet differed significantly from that of either control cell line or normal Beas-2B cells. A total of 409 differentially expressed genes were identified in chromate transformed cells compared to control cells.

2010-09-14 | GSE24025 | GEO

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