Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-MTAB-9077

ABSTRACT: RNA-seq of small intestinal organoids treated with an inhibitor for the epigenetic modifier LSD1

PROVIDER: E-MTAB-9077 | ExpressionAtlas | 2020-07-20

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-MTAB-9077-analysis-methods.tsv	Tabular
	E-MTAB-9077-analytics.tsv	Tabular
	E-MTAB-9077-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-9077-configuration.xml	Xml
	E-MTAB-9077-g2_g1-mvaPlot.png	Other

Items per page:

1 - 5 of 21

Similar Datasets

3a1d17cb-3261-4aab-923d-15ce6e5e8826 - samples

Project description:This dataset represent the RNA-seq, which was done on untreated small intestinal organoids; small intestinal organoids treated with chemotherapeutic, busulfan; untreated small intestinal organoids co-cultured wth mesenchymak stromal/stem cells (MSCs; busulfan treated small intestinal organoids co-cultured with MSCs. The same set of samples was done for 3 different primary bone marrow MSC donors.

| EGAD00001011176 | EGA

RNA-seq of small intestinal organoids treated with an inhibitor for the epigenetic modifier LSD1

Project description:RNA-seq of small intestinal organoids treated with an inhibitor for the epigenetic modifier LSD1

| PRJEB38358 | ENA

RNA-seq of small intestinal organoids treated with an inhibitor for the epigenetic modifier LSD1

Project description:To assess the role of LSD1 in mice small intestinal epithelium, small intestinal organoids were treated with an inhibitor for LSD1 (GSK-LSD1) and compared to untreated organoids. Similar to intestinal epithelium from mice with an intestinal epithelium specific LSD1-KO, paneth cells dissappear upon GSK-LSD1 treatment. We used these sequencing data to show that these small intestinal organoids have a similar phenotype as mice epithelium without LSD1.

2020-11-16 | E-MTAB-9077 | biostudies-arrayexpress

Human small intestinal organoids grown with a specific protocol to enrich for Paneth cells were treated with an inhbitor (GSK-LSD1) for the epigenetic modulator LSD1.

Project description:To assess the role of LSD1 in human intestinal epithelium, human small intestinal organoids were treated with an inhibitor for LSD1 (GSK-LSD1) and compared to untreated organoids. The organoids were grown with specific conditions where Paneth cells are present in the organoids as similar experiments in mice show that Paneth cells disappear upon GSK-LSD1 treatment. Similar to mouse intestinal organoids, Paneth cells dissappear upon GSK-LSD1 treatment. Furthermore, we used these gene set enrichment analysis on these microarray data to show that these human intestinal organoids have a similar phenotype as mice epithelium without LSD1.

2020-11-16 | E-MTAB-7863 | biostudies-arrayexpress

RNA-seq of mouse small intestinal organoids treated with interferon-gamma

Project description:RNA-seq of C57BL/6 derived small intestinal organoids treated with interferon-gamma. Control group represents non-treated group.

2021-01-21 | E-MTAB-9756 | biostudies-arrayexpress

Cell-autonomous transformation of Lgr5-positive intestinal stem cells into gastric stem cells upon loss of transcription factor Cdx2

Project description:Stomach and intestinal adult epithelia harbor stem cells that are responsible for their continuous regeneration. Stomach and intestinal stem cells differ in their differentiation program and in the gene repertoire that they express. We show that single adult Lgr5-positive stem cells, isolated from 3D cultured small intestinal organoids, require Cdx2 to maintain their intestinal identity and are converted cell-autonomously into stomach-pyloric stem cells in the absence of this transcription factor. In order to obtain Cdx2null intestinal stem cells carrying the Lgr5-EGFP marker, 5-6 days old small intestinal organoids generated from Cdx2-/fl/Lgr5-EGFP-Ires-CreERT2 mice were incubated with 1 µM of 4-hydroxytamoxifen in intestinal culture medium for 16h to activate the Cre recombinase. Controls were 4-hydroxytamoxifen-untreated small intestinal (Control SI) and stomach (Control Sto) organoids issued from mice with the same genotype. The organoids were dissociated and sorted for EGFPhi. Cdx2null, Control SI and Control Sto clonal organoids were generated and expanded from Lgr5-EGFPhi single cells in stomach specific culture medium (ENRWfg) and RNA was isolated for RNA-Seq analysis. Cdx2+ Stomach (Sto) organoids were generated by infection of the wild type stomach organoids with lentiviral stock expressing Cdx2. They were cultured in stomach medium (ENRWfg) and RNA was isolated for RNA-Seq analysis

2014-12-05 | E-GEOD-62784 | biostudies-arrayexpress

Expression data from intestinal organoids altered for Tgfbeta signaling

Project description:We treated intestinal organoids continuously for 5 days with or without TgfbR1/2 inhibitor (LY2109761) or Tgfb1 ligand Organoids were continuously treated for 5 days, then RNA was extracted and hybridized to Affymetrix Mouse Genome 430 2.0

2014-06-09 | E-GEOD-58296 | biostudies-arrayexpress

single-cell RNA sequencing for enteroids treated with IL-17

Project description:To evaluate the intestinal epithelial responses induced by IL-17, single-cell RNA-sequencing (scRNA-seq) was performed on the human small intestinal organoids (enteroids) sample of treated with and without 100 mg/ml IL-17

2021-08-20 | GSE182451 | GEO

RNA-seq of mouse small intestinal organoids treated with interferon-gamma

Project description:RNA-seq of mouse small intestinal organoids treated with interferon-gamma

| PRJEB41232 | ENA

RNA-seq of murine small intestinal organoids treated with type I PRMT inhibitor MS023 against untreated controls

Project description:RNA-seq of murine small intestinal organoids treated with type I PRMT inhibitor MS023 against untreated controls

| PRJEB39183 | ENA