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E-MTAB-9129

ABSTRACT: RNA-Seq of transgenic rice (Oryza sativa spp. japonica cultivar Kitaake) plants containing a single construct that over-expresses C4 metabolic pathway components CA, PEPC, MDH, ME and PPDK

PROVIDER: E-MTAB-9129 | ExpressionAtlas | 2023-06-16

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-9129-analysis-methods.tsv	Tabular
	E-MTAB-9129-analytics.tsv	Tabular
	E-MTAB-9129-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-9129-configuration.xml	Xml
	E-MTAB-9129-g2_g1-mvaPlot.png	Other

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RNA-Seq of transgenic rice (Oryza sativa spp. japonica cultivar Kitaake) plants containing a single construct that over-expresses C4 metabolic pathway components CA, PEPC, MDH, ME and PPDK

Project description:Transcriptome sequencing of transgenic and wild type plants to assess the patterns of altered gene expression associated with over expression of the 5 core C4 cycle metabolic pathway components – CA, PEPC, MDH, ME and PPDK.

2022-05-28 | E-MTAB-9129 | biostudies-arrayexpress

RNA-Seq of transgenic rice (Oryza sativa spp. japonica cultivar Kitaake) plants containing a single construct that over-expresses C4 metabolic pathway components CA, PEPC, MDH, ME and PPDK

Project description:RNA-Seq of transgenic rice (Oryza sativa spp. japonica cultivar Kitaake) plants containing a single construct that over-expresses C4 metabolic pathway components CA, PEPC, MDH, ME and PPDK

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A mechanistic linkage between low CO2 and evolution of C4 photosynthesis

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Project description:In order to change assimilate partitioning during seed maturation a bacterial phosphoenolpyruvate carboxylase (PEPC) has been expressed in V. narbonensis under control of the LegB4 promoter. Transgenic bean seeds accumulate app. 20% more protein and have higher seed weight. Physiological and biochemical analyses indicate a shift of metabolic fluxes from sugars/starch to organic acids/free amino acids suggesting increased anaplerotic carbon flow. This indicates that the availability of carbon acceptors limits seed protein synthesis. Gene expression was analysed in growing embryos using macro-arrays containing 5,548 seed-specific pea genes. Radioactive labeled cDNA probes were prepared from RNA isolated from embryo of developing seeds of wild type and transgenic PEPC plants of V. narbonensis (15, 17, 19, 21, 25, and 30 DAP) and hybridized to cDNA macroarrays.

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Project description:Transcriptome analysis of C4-pepc gene transgenic rice to low nitrogen

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