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E-MTAB-9932

ABSTRACT: Time-course transcriptome analysis in roots of N-limited Medicago truncatula plants grown in split-root inoculated with Sinorhizobium medicae

PROVIDER: E-MTAB-9932 | ExpressionAtlas | 2021-01-15

REPOSITORIES: ExpressionAtlas

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Time-course transcriptome analysis in roots of N-limited Medicago truncatula plants grown in split-root inoculated with Sinorhizobium medicae

Project description:Time-course transcriptome analysis in roots of N-limited Medicago truncatula plants grown in split-root inoculated with Sinorhizobium medicae

| PRJEB42176 | ENA

E-MTAB-8168

Project description:Time course analysis of the cardiac transcriptome in the neonatal period

2019-08-07 | E-MTAB-8168 | ExpressionAtlas

Time-course transcriptome analysis in roots of N-limited Medicago truncatula plants grown in split-root inoculated with Sinorhizobium medicae

Project description:The experiment aims to analyze the effect of inoculation with Sinorhizobium medicae MD4 of on the transcriptome of the Medicago truncatula roots of N-limited plants. We divided the roots systems into two parts; one of them was supplied with nutrient solution with mineral N (0.5 mM for N-limited plants) whereas the non-N treated part was kept in nutrient solution without mineral N. All roots were inoculated 48h after initiation of the N treatments. We collected the roots the non-N treated side of the split roots system and we isolated total RNA for RNAseq analysis. Root transcriptomes were analyze before inoculation, at 1 and 2 days post-inoculation.

2021-04-30 | E-MTAB-9932 | biostudies-arrayexpress

Effect of contrasted N regimes on the root transcriptome of Medicago truncatula plants cultivated in split root systems and inoculated for 2, 4 and 7 days with Sinorhizobium medicae

Project description:The experiment aims to analyze the effect of systemic signaling of plant N demand on the transcriptome of Medicago truncatula roots inoculated with Sinorhizobium medicae MD4 at 2, 4, and 7 days post-inoculation (dpi). We divided the roots systems into two parts; one of them was supplied with nutrient solution with mineral N (10 mM NH4NO3 for N-satisfied plants, 0.5 mM for N-limited plants) whereas the non-N treated part was kept in nutrient solution without mineral N. All roots were inoculated 48h after initiation of the N treatments. To characterize specifically the impact of systemic signaling of N demand, we collected the roots the non-N treated sides of the split roots systems and we isolated total RNA for RNAseq analysis. Root transcriptomes of N-limited and N-satisfied plants were compared at 2, 4 and 7 dpi.

2021-04-30 | E-MTAB-9941 | biostudies-arrayexpress

E-GEOD-37161

Project description:Transcription profiling by array of Nipponbare rice roots over a time course of potassium deprivation

2014-07-03 | E-GEOD-37161 | ExpressionAtlas

Effect of the sunn mutation on systemic signalling of the plant N demand in Medicago truncatula plants inoculated for 2 or 7 days with Sinorhizobium medicae

Project description:The experiment aims to investigate the effect of the sunn mutation on systemic signaling of the plant N demand in Medicago truncatula roots inoculated with Sinorhizobium medicae MD4 at 2 and 7 days post-inoculation (dpi). We compared the TR122/sunn-2 mutant to the wild type Jemalong A17. For each plant genotype, we divided the roots systems into two parts; one of them was supplied with a nutrient solution with mineral N (10 mM NH4NO3 for N-satisfied plants, 0.5 mM for N-limited plants) whereas the non-N treated part was kept in a nutrient solution without mineral N. All roots were inoculated 48h after initiation of the N treatments. To characterize specifically the impact of systemic signaling of N demand, we collected for both genotypes the roots of the non-N treated sides of the split roots systems, and we isolated total RNA for RNAseq analysis. Root transcriptomes of N-limited and N-satisfied plants were compared in both genotypes at 2 or 7 dpi.

2021-04-30 | E-MTAB-9942 | biostudies-arrayexpress

Time-course transcriptome analysis of mature nodules in Medicago truncatula grown in split-root under different nitrogen (N) supply

Project description:The experiment aim is the identification of the transcriptional response of mature nodules of Medicago truncatula inoculated with the strain MD4 of Sinorhizobium medicae in response to different nitrogen (N) supply (N-satiety and N-deficient conditions). We performed a time-course (1 and 3 days treatment) split-root experiment to identify plant systemic responses: the root systems of nodulated symbiotic plants were divided into two compartments, one of them treated and the other kept on control conditions. Nodules were isolated from the non-treated side of the split root and total RNA isolated for RNAseq analysis.

2020-09-30 | E-MTAB-8597 | biostudies-arrayexpress

Time-course transcriptome analysis of Sinorhizobium medicae (MD4 strain) in mature nodules of Medicago truncatula grown in split-root under different nitrogen (N) supply

2020-09-30 | E-MTAB-8598 | biostudies-arrayexpress

Time-course transcriptome analysis of mature nodules in Medicago truncatula grown in split-root under different nitrogen (N) supply

Project description:Time-course transcriptome analysis of mature nodules in Medicago truncatula grown in split-root under different nitrogen (N) supply

| PRJEB35811 | ENA

Transcript analysis of early nodulation events in Medicago truncatula

Project description:For transcript analysis of early nodulation events in Medicago truncatula we compared transcripts from inoculated and uninoculated roots corresponding to defined stages between 1 and 72 h post inoculation (hpi). Keywords: time course

2005-10-14 | GSE3441 | GEO

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