Project description:The MFP2-/- (multifunctional protein 2) is a multifunctional enzyme with discovery in connection with different metabolic pathways. It is involved in beta-oxidation of branched fatty acids and long chain fatty acids in peroxisomes and in sythesis of bile fatty acids. MFP2 knock out mouse was made with a phenotype only visible in homozygotes (severe growth retardation, 40% mortality during the first week after birht, reduction of fertility in males, less activity). The studies have been performed to identify changes in gene expression levels in kidney due to clinical chemical changes in the phenotype of this mutant mouse line during a screening in the German Mouse Clinic. Keywords: dual colour hybridisation on cDNA microarrays, MFP2, Hsd17b4, kidney
Project description:Glucagon is a key regulator of glucose homeostasis, amino acid catabolism, and lipid metabolism. Glucagon receptor knock-out (GcgrKO) mice have slightly reduced blood glucose levels whereas plasma levels of amino acids are vastly increased reflecting disruption of hepatic amino acid catabolism. To dissect the molecular mechanisms underlying this effect, RNA sequencing of livers from male GcgrKO mice and wild-type littermates were performed. The mice were 10 weeks of age and were subjected to a short-term fast of 4 h before anesthesia with 2.5% isoflurane.
Project description:We generated iPS cells knock out (KO) for two Aicardi Goutières syndrome genes, TREX1 and RNASEH2b to model the disease in a human neurological context. However, it has been reported that after precise genome engineering human iPSCs may show severe alterations of the p53 gene. With the aim to evaluate the genome engeneering impact on the p53 pathway in the CRISPR-CAS9 knock out clones we verified integrity of the p53 genome sequence after gene editing.
Project description:HEK293T proteome (single shot) with knock-in or not for RPS3-Halo and Knock out or not for NUFIP1. Cells were treated or not with Torin1 or deprived of amino acids.
Project description:We generated knock-in mice that express wildtype human Aβ under control of the mouse App locus. Remarkably, changing 3 amino acids in the mouse Aβ sequence to its wild-type human counterpart leads to age-dependent impairments in cognition and synaptic plasticity, brain volumetric changes, inflammatory alterations, the appearance of Periodic Acid-Schiff (PAS) granules and changes in gene expression.
Project description:β-cell specific Mettl14 knock-out mice display reduced N6-methyladenosine (m6A) levels and recapitulate human Type II diabetes (T2D) islet phenotype with early diabetes onset and mortality secondary to decreased β-cell proliferation and insulin degranulation. To gain insights into the role of m6A in regulating the IGF1/insulin -> AKT - > PDX1 pathway and to dissect the signaling networks modulating AKT phosphorylation, we subjected freshly isolated islets from control and Mettl14 knock-out mice to phospho-antibody microarrays.
Project description:Amino acids conjugated with even number carbon fatty acids using a coupling of the chloride fatty acids onto amine end of amino acids
Project description:In search for peptides with higher or special binding affinity and for further understanding of the mode of action, a full substitutional analysis of peptide PeB using microarrays was performed. Thus, 152 PeB mutant variants were generated. In each of them, the full-length sequence was preserved except for only one amino acid from the eight loop-forming amino acids of the original PeB peptide (ARDFYDYDVFYYAMD) which was substituted with the 19 remaining natural amino acids. To assess binding, influenza material was labeled with a protein reacting fluorophore.
Project description:Opn3 knock out mice show transcriptional changes in these tissues that are indicative of deregulation of mitochondrial function, extracellular matrix and growth factor signaling, fatty acid oxidation and uptake defects.
Project description:The MFP2-/- (multifunctional protein 2) is a multifunctional enzyme with discovery in connection with different metabolic pathways. It is involved in beta-oxidation of branched fatty acids and long chain fatty acids in peroxisomes and in sythesis of bile fatty acids. MFP2 knock out mouse was made with a phenotype only visible in homozygotes (severe growth retardation, 40% mortality during the first week after birht, reduction of fertility in males, less activity). The studies have been performed to identify changes in gene expression levels in kidney due to clinical chemical changes in the phenotype of this mutant mouse line during a screening in the German Mouse Clinic. Keywords: dual colour hybridisation on cDNA microarrays, MFP2, Hsd17b4, kidney DNA chip technology has been exploited for RNA expression profiling analyses. Close to genomewide microarrays were used for identification of changes in gene expression levels in kidney of 4 MFP2-/- mice. Individual mutant mice were hybridised in repetitions against a pool of 5 reference animals. In total 16 chip hybridisations were performed including 50% dye swap experiments.