Two distinct waves of transcriptome and translatome changes drive germline stem cell differentiation

ABSTRACT: The tight control of fate transitions during stem cell differentiation is essential for proper tissue development and maintenance. However, identifying how the multilayered regulation of gene expression programmes orchestrates stem cell differentiation in vivo remains elusive, because of the challenges in studying sparsely distributed adult stem cells in a systematic manner. Here, we synchronised Drosophila female germline stem cell (GSC) differentiation in vivo to perform in-depth transcriptome and translatome analyses at high developmental resolution. This characterisation revealed widespread and dynamic changes in mRNA level, promoter usage, exon inclusion, and translation efficiency. Transient expression of the master regulator, Bam, drives a first wave of expression changes, primarily

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PROVIDER: S-BIAD1009 | bioimages |

REPOSITORIES: bioimages