Small ADP-ribosylation factor-like GTPase TITAN 5 (TTN5/ARL2) is linked with the dynamic regulation of IRON-REGULATED TRANSPORTER 1

ABSTRACT: Iron acquisition is crucial for plants. The abundance of IRON-REGULATED TRANSPORTER 1 (IRT1) at the plasma membrane is controlled through endomembrane trafficking. Vesicular trafficking requires small ARF-like GTPases, one of them is TITAN 5 (TTN5). Its physiological functions during the life cycle and effector targets remain unknown. Little is known how the vesicular trafficking mechanism controls IRT1. We show that TTN5 interacts with the large cytoplasmic variable region and protein-regulatory platform of IRT1. ttn5-1+/- plants have a reduced activity of root iron reductase, closely associated with iron uptake. Fluorescent fusion proteins of TTN5 and IRT1 colocalize at the plasma membrane and in endosomes/multivesicular bodies, where IRT1 sorting and cycling between the plasma membran

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PROVIDER: S-BIAD1351 | bioimages |

REPOSITORIES: bioimages