DNA methylation modulates nucleosome retention in sperm and H3K4 methylation deposition in early mouse embryos.

ABSTRACT: DNA methylation (DNAme) serves a stable gene regulatory function in somatic cells. In the germ line and during early embryogenesis, however, DNAme undergoes global erasure and re-establishment to support germ cell and embryonic development. While de novo DNAme acquisition during male germ cell development is essential for setting genomic DNA methylation imprints, other intergenerational roles for paternal DNAme in defining embryonic chromatin after fertilization are unknown. To approach this question, we reduce levels of DNAme in developing male germ cells through conditional gene deletion of the de novo DNA methyltransferases DNMT3A and DNMT3B in undifferentiated spermatogonia. We observe that DNMT3A primarily safeguards against DNA hypomethylation in undifferentiated spermatogonia, while

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PROVIDER: S-BIAD1457 | bioimages |

REPOSITORIES: bioimages