Cell-autonomous timing drives the vertebrate segmentation clock’s wave pattern

ABSTRACT: Rhythmic and sequential segmentation of the growing vertebrate body relies on the segmentation 20 clock, a multi-cellular oscillating genetic network. The clock is visible as tissue-level kinematic waves of gene expression that travel through the presomitic mesoderm (PSM) and arrest at the position of each forming segment. Here we test how this hallmark wave pattern is driven by culturing single maturing PSM cells. We compare their cell-autonomous oscillatory and arrest dynamics to those we observe in the embryo at cellular resolution, finding similarity in the relative 25 slowing of oscillations and arrest in concert with differentiation. This shows that cell-extrinsic signals are not required by the cells to instruct the developmental program underlying the wave pattern. We show that a c

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PROVIDER: S-BIAD1500 | bioimages |

REPOSITORIES: bioimages