Spatio-temporal analysis of the innate immune response to cytoplasmic dsDNA and microenvironmental cGAMP using a novel STING-IRF3 biosensor

ABSTRACT: The cGAS-STING signalling pathway acts as gatekeeper in the innate immune response towards extrinsic and intrinsic sources of cytoplasmic double stranded DNA (dsDNA). At the core of this pathway is the cGAS-dependent production of the intra- and extra-cellular messenger 2’3’-cyclic GMP-AMP (cGAMP), which activates STING and leads to IRF3-dependent expression of cytokines and interferon. Despite its relevance to monitor viral and bacterial infections, cell death, and genome instability, the lack of specific live reporters has precluded spatio-temporal analyses of cGAS-STING signalling. Here, we generate a fluorescent biosensor by engineering the functional interaction of activated STING and IRF3 at the Golgi, which we termed SIRF (STING-IRF3). We show that cells encoding for the SIRF biosen

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PROVIDER: S-BIAD1536 | bioimages |

REPOSITORIES: bioimages