Quantitative 3D Imaging of the Cranial Microvascular Environment at Single-Cell Resolution

ABSTRACT: Vascularization is critical for skull development, maintenance, and healing. Yet, there remains a significant knowledge gap in the relationship of blood vessels to cranial skeletal progenitors during these processes. Here, we introduce a quantitative 3D imaging platform to enable visualization and analysis of high-resolution datasets (>100 GB) throughout the entire murine calvarium. Using this technique, we provide single-cell resolution 3D maps of vessel phenotypes and skeletal progenitors in the frontoparietal cranial bones. Through these high-resolution datasets, we demonstrate that CD31hiEmcnhi vessels are spatially correlated with both Osterix+ and Gli1+ skeletal progenitors during postnatal growth, healing, and stimulated remodeling, and are concentrated at transcortical canals and o

ORGANISM(S): Mus musculus (mouse)

SUBMITTER: Alexandra N. Rindone 

PROVIDER: S-BIAD171 | bioimages |

REPOSITORIES: bioimages