Photon Absorption Remote Sensing Virtual Histopathology: A Preliminary Exploration of Diagnostic Equivalence to Gold-Standard H&E Staining in Skin Cancer Excisional Biopsies

ABSTRACT: Photon Absorption Remote Sensing (PARS) enables label-free imaging of subcellular morphology by observing biomolecule specific absorption interactions. Coupled with deep-learning, PARS produces label-free virtual Hematoxylin and Eosin (H&E) stained images in unprocessed tissues. This set of example of PARS virtual H&E images is from a clinical study available at https://doi.org/10.48550/arXiv.2504.18737. The purpose of this study is to evaluate the diagnostic performance of these PARS-derived virtual H&E images in benign and malignant excisional skin biopsies, including Squamous (SCC), Basal (BCC) Cell Carcinoma, and normal skin. Sixteen unstained formalin-fixed paraffin-embedded skin excisions were PARS imaged, virtually H&E stained, then chemically stained and imaged at 40x. Seven fellowship trained dermatopathologists assessed all 32 images in a masked randomized fashion. A subset of 3 PARS and 3 chemical H&E (BCC, SCC, and benign) are presented here.

SUBMITTER: Benjamin Ecclestone 

PROVIDER: S-BIAD2324 | bioimages |

REPOSITORIES: bioimages