Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events

ABSTRACT: The rapid spread of antimicrobial resistance and vaccine escape in the opportunistic human pathogen Streptococcus pneumoniae can be largely attributed to competence-induced transformation. To better understand the dynamics of competence-induced transformation, we studied this process at the single-cell level. We show that within isogenic populations, all cells become naturally competent and bind exogenous DNA. In addition, we find that transformation is highly efficient and that the chromosomal location of the integration site or whether the transformed gene is encoded on the leading or lagging strand has limited influence on recombination efficiency. Indeed, we have observed multiple recombination events in single recipients in real-time. However, because of saturation of the DNA uptake a

SUBMITTER: Jan-Willem Veening 

PROVIDER: S-BIAD26 | bioimages |

REPOSITORIES: bioimages