Marine Broth induces extreme morphological transformations in Chlamydomonas smithii

ABSTRACT: Chlamydomonas smithii exhibits remarkable morphological plasticity (pleomorphism) in response to environmental conditions. This dataset captures the diverse cell shapes and subcellular structures observed when C. smithii cultures are maintained under nutrient-depleted conditions over extended periods (40-43 days). We examined cells grown on multiple media types (Marine Broth, Erdschreiber's Medium, Kuhl's Medium, TAP, and water) supplemented with 1.5% agar, resuspended in liquid growth media, and applied various fluorescent stains (FM4-64 membrane stain, MitoTracker Orange, PKmito Orange) to visualize subcellular compartments. The dataset includes brightfield, RGB, and fluorescence z-stack images capturing the wide range of pleomorphic forms that emerge during prolonged culture, including multi-lobed cells, enlarged vacuoles, and altered chloroplast morphologies.

SUBMITTER: Cameron MacQuarrie 

PROVIDER: S-BIAD2873 | bioimages |

REPOSITORIES: bioimages