Detecting chromatin state alterations in PBMCs associated with Type 2 Diabetes Mellitus

ABSTRACT: In this study, we examined blood samples from 57 people, including healthy individuals, people with prediabetes, and people with type 2 diabetes (T2DM). Using a microscopy-based method to look at how DNA is organized inside immune cells, we found clear differences between these groups. We observed that certain immune cell types show distinct changes as diabetes progresses. These changes were specific to individual patients and were more pronounced in people with T2DM compared to those with prediabetes or no diabetes. In people with T2DM, immune cells also showed signs of increased activation, reduced levels of a structural protein called Lamin, and altered mechanical properties, suggesting that the cells are under stress and behaving differently. Overall, our results show that chromatin imaging can capture meaningful changes in immune cells linked to diabetes progression. Because this approach is relatively affordable and scalable, it has potential to be used in clinical settings to help monitor disease progression and support more personalized management of type 2 diabetes.

SUBMITTER: Rajshikhar Gupta 

PROVIDER: S-BIAD2905 | bioimages |

REPOSITORIES: bioimages