Multispectral imaging of 8 organelles in live undifferentiated hiPSCs and iNeurons at day7, 14, 21 and 28

ABSTRACT: Live undifferentiated KOLF2.1J human iPSCs and iNeurons, were labelled with 8 organelle markers targeted to the nucleus*, endoplasmic reticulum (ER), Golgi, mitochondria, peroxisomes, lysosomes, lipid droplets and plasma membrane. Complete z-stack and timelapse images were acquired in lambda mode (410-695 nm, 8.9 nm resolution) and subsequently linear unmixed into 9 different channels (channel 1 to 8 correspond to organelles, while channel 9 is the residual of the unmixing). Images were next deconvolved using Huygens Essential software version 23.04 (Scientific Volume Imaging, The Netherlands, http://svi.nl). Next, using Infer-subc imaging pipeline (https://github.com/SCohenLab/infer-subc/blob/v2.0.0b1), images were segmented to obtain organelle segmentations, and cell region for iPSCs, or soma/neurites regions for iNeurons. These data were used in the following preprint (https://doi.org/10.64898/2026.02.10.704675)

SUBMITTER: Maria Clara Zanellati 

PROVIDER: S-BIAD2983 | bioimages |

REPOSITORIES: bioimages