CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies

ABSTRACT: Cell-cycle progression is a major source of variability in live-cell phenotyping, yet imaging workflows still lack a general way to account for it alongside structural and functional readouts. This study presents CALIPERS (Cell-cycle-Aware Live-cell Imaging for Phenotyping Experiments and Regeneration Studies), an integrated framework for cell-cycle-aware live imaging built around a spectrally redesigned FUCCI reporter, continuous cell-cycle phase inference, and flexible (lentiviral and safe-harbor) delivery strategies in human cell and stem-cell models. The redesigned four-colour reporter preserves the widely used GFP/RFP biosensor ecosystem, allowing cell-cycle state to be imaged simultaneously with structural and functional readouts. In human epithelial cells (HaCaT), CALIPERS enables s

SUBMITTER: Moises Di Sante 

PROVIDER: S-BIAD3686 | bioimages |

REPOSITORIES: bioimages