Uncovering the mode of action of engineered T cells in patient cancer organoids

ABSTRACT: The rise of numerous promising immunotherapies poses a need for technology platforms to study their efficacy and mechanism of action. Organoids are cellular 3D structures that recapitulate important tumour characteristics and there is growing interest to use such technology to evaluate immunotherapy efficacy. Imaging provides a powerful approach to probe the cellular complexity modelled with organoids, but requires the development of adequate analytical methodology to comprehensively investigate the large and complex datasets retrieved. Here, we developed an organoid-based 3D live cell imaging and data mining platform and applied it to TEGs (T cells engineered to express a V9/V2 T cell receptor (TCR)) that sense metabolic changes via the recently identified ligand butyrophilin 2A1 (BTN

ORGANISM(S): Homo sapiens (human)

SUBMITTER:  

PROVIDER: S-BIAD448 | bioimages |

REPOSITORIES: bioimages