Diffusion and bulk flow of amino acids mediate calcium waves in plants

ABSTRACT: In plants, a variety of stimuli trigger long-range calcium signals that travel rapidly along the vasculature to distal tissues via poorly understood mechanisms. Here, we use quantitative imaging and analysis to demonstrate that travelling calcium waves are mediated by diffusion and bulk flow of amino acid chemical messengers. Wounding triggers release of amino acids that diffuse locally through the apoplast, activating the calcium permeable channel GLUTAMATE-LIKE RECEPTOR3.3 as they pass. Over long distances through the vasculature, the wound-triggered dynamics of a fluorescent tracer show that calcium waves are likely driven by bulk flow of a channel activating chemical. We observed that multiple stimuli trigger calcium waves with similar dynamics, but calcium waves alone cannot initiate

ORGANISM(S): Arabidopsis thaliana (thale cress)

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PROVIDER: S-BIAD464 | bioimages |

REPOSITORIES: bioimages