Monitoring drug-induced signaling inhibition and adaptive resistance by live-cell microscopy

ABSTRACT: Genetically identical cells that are experiencing similar extracellular conditions can still be phenotypically distinct from one another. Phenotypic variation in key traits can impact cellular fitness and have clinical importance, as has been reported for variation in bacterial persistence during antibiotic treatment and variation in drug resistance among cancer cells. Cell-cell variability is typically studied with methods amenable to high-throughput measurements such as flow-cytometry, yet these methods are unsuitable for monitoring dynamic phenotypes that change and evolve over time. Cellular signaling represents an important class of dynamic phenotypes that remain inaccessible for exploration with end-time point measurements. Here, we describe an experimental and computational pipeline

ORGANISM(S): Homo sapiens (human)

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PROVIDER: S-BIAD478 | bioimages |

REPOSITORIES: bioimages