B1-integrin binding and lysosome targeting aptamer homes to triple-negative breast cancer in mice (in vivo images)

ABSTRACT: 2 nmol of GreenB1-FAM or RND-FAM were injected i.v. (n=3 in each group). After one hour of circulation time, mice were anaesthetised, sacrificed, perfused, and tumour, heart, liver, lung, and kidney were collected. Organs were flash-frozen in liquid nitrogen and kept at -80 °C until further use. Organs were sectioned at 30 um and fixed in methanol (equilibrated at -20 °C) for 2 minutes. 5%BSA in PBS with 0.05% Tween-20 was applied to tissue sections for 30 min at room temperature to block non-specific antibody binding. GreenB1-FAM or RND-FAM was detected using a 1:200 dilution of rabbit anti-fluorescein antibody (#A-11090, ThermoFisher) at room temperature for one hour, followed by 1:400 dilution of secondary anti-rabbit AlexaFluor555 labelled antibody (#A32794, ThermoFisher) at room tempe

ORGANISM(S): Mus musculus (mouse)

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PROVIDER: S-BIAD483 | bioimages |

REPOSITORIES: bioimages