In situ nanopore sequencing to study coral holobionts

ABSTRACT: Molecular characterisation of the coral host and its associated microbial assemblages (referred to as the coral holobiont) is currently undertaken via marker gene sequencing. Recent advances in sequencing technologies now permit rapid sequencing in the field. However, development of specific protocols and pipelines for the effective processing of complex microbial systems are currently lacking. Here, we chose a combination of 3 marker genes targeting the coral animal host, its symbiotic alga, and the associated bacterial microbiome, to characterize 60 coral colonies collected and processed in situ, onboard of the research vessel Tara during the Tara Pacific expedition. We used Oxford Nanopore Technologies (ONT) to sequence marker gene amplicons and developed bioinformatics pipelines to ana

SUBMITTER: Quentin Carradec 

PROVIDER: S-BIAD5 | bioimages | 2020-01-01

REPOSITORIES: bioimages