Self-assembled endogenous DNA nanoparticles for auto-release and expression of the eGFP gene in Bacillus subtilis

ABSTRACT: In this study, the modified DAEDALUS module, DX_cage_design, was used to design a 50.7 nm nanocube by reading in the sequence of citZ gene, encoding citrate synthase of B. subtilis 168, and circulating it nine times with a remainder function, rem (edge_length, 21), ensuring to the predicted structure as B-type DNA29. The sequence of citZ, used as scaffold strand, was folded nine times to form deoxynucleotide nanoparticles by multimodular self-assembly, named a citZ-box. The scaffold strands were elongated to 9984 nt, which involved seven 1116 nt scaffolds and two short main scaffold strands of 812 nt and 1034 nt, respectively. Atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) revealed a nanocube with an average edge of 50.3 ± 0.3

ORGANISM(S): Nucleic acids

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PROVIDER: S-BIAD581 | bioimages |

REPOSITORIES: bioimages