SLE Serum Induces Altered Goblet Cell Differentiation and Leakiness in Human Intestinal Organoids

ABSTRACT: Human intestinal epithelial cells are the interface between luminal content and basally residing immune cells. They form a tight monolayer that constantly secretes mucus creating a multi-layered protective barrier. Alterations in this barrier can lead to increased permeability which is common in Systemic Lupus Erythematosus (SLE) patients. However, it remains unexplored how the barrier is affected. Here, we present an in vitro model specifically designed to examine the effects of SLE on epithelial cells. We utilize human colon organoids that are stimulated with serum from SLE patients. Combining transcriptomic with functional analyses revealed that SLE serum induced an expression profile marked by a reduction of goblet cell markers and changed mucus composition. Additionally, organoids exh

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PROVIDER: S-BIAD978 | bioimages |

REPOSITORIES: bioimages