Differential roles of AVP and VIP signaling in the postnatal changes of neural networks for coherent circadian rhythms in the SCN

ABSTRACT: Cry double deficient (Cry1,2?/?) mice and Vip receptor 2 deficient (Vipr2?/?) mice were bred with PER2::LUC mice carrying a PER2 luciferase reporter. Wild–type (Cry1,2+/+/Vipr2+/+) PER2::LUC transgenic mice on the C57BL/6J background were used as control. For the measurement of PER2::LUC bioluminescence from a cultured SCN slice, mice of 8–16 weeks or 2–5 days old, kept under LD condition, were euthanized between 8:00 and 16:00 by cervical dislocation and decapitated. The brain was rapidly removed and a coronal SCN slice of 150 ?m or 200 ?m was made. In the co–culture experiment, the SCN slices of 150 ?m thick were obtained from adult mice carrying the PER2::LUC reporter (recipient). The slice was pre-cultured for 3 or 4 days, and then co–cultured with an SCN slice from mice without the re

SUBMITTER: Daisuke Ono 

PROVIDER: S-BSST212 | bioimages |

REPOSITORIES: bioimages