The Subcellular Proteome of a Planctomycetes Bacterium Shows that Newly Evolved Proteins have Distinct Biochemical Characteristics

ABSTRACT: The Planctomycetes bacteria have unique cell architectures with heavily invaginated membranes as confirmed in this study by three-dimensional models reconstructed from FIB-SEM images of Tuwongella immobilis and Gemmata obscuriglobus. The subcellular proteome of T. immobilis was examined by differential solubilisation followed by LC-MS/MS analysis, which identified 1569 proteins in total. The Tris-soluble fraction contained mostly cytoplasmic proteins, while inner and outer membrane proteins were found in the Triton X-100 and SDS-soluble fractions, respectively. For comparison, the subcellular proteome of Escherichia coli was also examined using the same methodology. A notable difference in the overall fractionation pattern of the two species was a 5-fold higher number of cytoplasmic protei

ORGANISM(S): Tuwongella immobilis, Gemmata obscuriglobus, Escherichia coli

SUBMITTER: Christian Seeger 

PROVIDER: S-BSST564 | bioimages |

REPOSITORIES: bioimages