Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging

ABSTRACT: Phytoplankton is a minor fraction of the global biomass playing a major role in primary production and climate. Despite improved understanding of phytoplankton diversity and genomics, we lack nanoscale subcellular imaging approaches to understand their physiology and cell biology. Here, we present a complete Focused Ion Beam - Scanning Electron Microscopy (FIB-SEM) workflow (from sample preparation to image processing) to generate nanometric 3D phytoplankton models. Tomograms of entire cells, representatives of six ecologically-successful phytoplankton unicellular eukaryotes, were used for quantitative morphometric analysis. Besides lineage-specific cellular architectures, we observed common features related to cellular energy management: i) conserved cell-volume fractions occupied by the

ORGANISM(S): Microalgae cell

SUBMITTER: Clarisse Uwizeye 

PROVIDER: S-BSST575 | bioimages |

REPOSITORIES: bioimages