Single-cell Transcriptomic Analysis of Oral Masticatory and Lining Mucosa-derived Mesenchymal Stromal Cells

ABSTRACT: Aims To reveal the heterogeneity of ex vivo-cultured human mesenchymal stromal cells derived from either masticatory or lining oral mucosa. Materials and methods Cells were retrieved from the lamina propria of the hard palate and alveolar mucosa of three individuals. The analysis of transcriptomic level differences was accomplished using single-cell RNA-sequencing. Results Cluster analysis clearly distinguished between cells from the masticatory and lining oral mucosa and revealed eleven distinct cell subpopulations, annotated as fibroblasts, smooth muscle cells, or mesenchymal stem cells. Interestingly, cells presenting a mesenchymal stem cell-like gene expression pattern were predominantly found in masticatory mucosa. While cells of masticatory mucosa origin were highly enriched for biol

ORGANISM(S): Homo sapiens (human)

SUBMITTER:  

PROVIDER: S-BSST929 | bioimages |

REPOSITORIES: bioimages