KLP-7 acts through the Ndc80 complex to limit pole number inC. elegansoocyte meiotic spindle assembly

ABSTRACT: During oocyte meiotic cell division in many animals, bipolar spindles assemble in the absence of centrosomes, but the mechanisms that restrict pole assembly to a bipolar state are unknown. We show that KLP-7, the single mitotic centromere–associated kinesin (MCAK)/kinesin-13 inCaenorhabditis elegans, is required for bipolar oocyte meiotic spindle assembly. Inklp-7(−)mutants, extra microtubules accumulated, extra functional spindle poles assembled, and chromosomes frequently segregated as three distinct masses during meiosis I anaphase. Moreover, reducing KLP-7 function in monopolarklp-18(−)mutants often restored spindle bipolarity and chromosome segregation. MCAKs act at kinetochores to correct improper kineto

SUBMITTER: Amy A. Connolly 

PROVIDER: S-JCBD-201412010 | bioimages |

REPOSITORIES: bioimages