Avian influenza is a zoonotic disease caused by the transmission of the avian influenza A virus, such as H5N1 and H7N9, from birds to humans. The avian influenza A H5N1 virus has caused more than 500 human infections worldwide with nearly a 60% death rate since it was first reported in Hong Kong in 1997. The four outbreaks of the avian influenza A H7N9 in China from March 2013 to June 2016 have resulted in 580 human cases including 202 deaths with a death rate of nearly 35%. In this paper, we co ...[more]
Project description:To clarify the underlying mechanism that regulates the response of iron metabolism to influenza A virus infection, we analyzed gene expression profiles in mouse, bone marrow-derived macrophages (BMDMs) infected with H7N9 virus. In addition, We also analysed the chemokine, inflammation, innate-immunity, lipid-metabolism, transcription mRNA level by micrroarray. So, we gain the global transcriptional response in the BMDMs of mice infected with H7N9 virus. Overall design: BMDMs were seperated from 4-6 week-old BALB/C mice (wild type, WT and Hfe KO) and inoculated with H7N9 virus for 6h and 12h, and the uninfected BMDMs were as a control.The cells were collected to RNA extraction and hybridization on Agilent microarrays. Samples were named as follows: Control (WT-1), treatment(WT-12h-2, WT-6h-34, KO-5, KO-6h-67, KO-12h-8).
Project description:Transcriptional profiling was carried out on lung and ileum samples at 1dpi and 3dpi from quail infected with either low pathogenic (H5N2) or highly pathogenic (H5N1) avian influenza. Infected birds were compared to control birds at each time point.