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Heiland2019 - NAD pathway model analysing the impact of NNMT on pathway dynamics and evolution

ABSTRACT: This is a detailed model of NAD biosynthesis and consumption representing pathway variances across kingdoms.

SUBMITTER: Ines Heiland

PROVIDER: MODEL1905220001 | BioModels | 2019-07-26

REPOSITORIES: BioModels

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Structural and kinetic isotope effect studies of nicotinamidase (Pnc1) from Saccharomyces cerevisiae.

Smith Brian C BC Anderson Mark A MA Hoadley Kelly A KA Keck James L JL Cleland W Wallace WW Denu John M JM

Biochemistry 20111229 1

Nicotinamidases catalyze the hydrolysis of nicotinamide to nicotinic acid and ammonia. Nicotinamidases are absent in mammals but function in NAD(+) salvage in many bacteria, yeast, plants, protozoa, and metazoans. We have performed structural and kinetic investigations of the nicotinamidase from Saccharomyces cerevisiae (Pnc1). Steady-state product inhibitor analysis revealed an irreversible reaction in which ammonia is the first product released, followed by nicotinic acid. A series of nicotina ...[more]

PMID: 22229411

Publication: 1/10

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