Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis roots with or without treatment with 9-hydroxyoctadecatrienoic acid

ABSTRACT: Gene expression of roots grown on MS medium versus MS+9HOT was compared. 6-day-old seedlings grown on MS were transferred to fresh plates and grown for 3- or 5- additional days in the absence or in the presence of 9-HOT (25 um). Root fragments grown after transferring to fresh plates were excised and used to compare gene expression between controls and 9-HOT treated seedlings.

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Marta Martinez 

PROVIDER: E-ATMX-14 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Oxylipins produced by the 9-lipoxygenase pathway in Arabidopsis regulate lateral root development and defense responses through a specific signaling cascade.

Vellosillo Tamara T   Martínez Marta M   López Miguel Angel MA   Vicente Jorge J   Cascón Tomas T   Dolan Liam L   Hamberg Mats M   Castresana Carmen C  

The Plant cell 20070316 3

Arabidopsis thaliana seedling growth with pure oxylipins resulted in root waving, loss of root apical dominance, and decreased root elongation. 9-Hydroxyoctadecatrienoic acid (9-HOT) was a potent inducer of root waving. Studies with noxy2 (for nonresponding to oxylipins2), a new 9-HOT-insensitive mutant, and coronatine insensitive1-1 (jasmonate-insensitive) revealed at least three signaling cascades mediating the oxylipin actions. Treatment with 9-HOT resulted in a reduction in lateral roots and  ...[more]

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