Transcription profiling of Arabidopsis cell cultures after Actinomycin D (transcriptional inhibitor) treatment to investigate global mRNA decay
Ontology highlight
ABSTRACT:
INSTRUMENT(S): GeneChip Scanner 3000 [Affymetrix]
ORGANISM(S): Arabidopsis thaliana
SUBMITTER: Reena Narsai
PROVIDER: E-ATMX-21 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Genome-wide analysis of mRNA decay rates and their determinants in Arabidopsis thaliana.
Narsai Reena R Howell Katharine A KA Millar A Harvey AH O'Toole Nicholas N Small Ian I Whelan James J
The Plant cell 20071116 11
To gain a global view of mRNA decay in Arabidopsis thaliana, suspension cell cultures were treated with a transcriptional inhibitor, and microarrays were used to measure transcript abundance over time. The deduced mRNA half-lives varied widely, from minutes to >24 h. Three features of the transcript displayed a correlation with decay rates: (1) genes possessing at least one intron produce mRNA transcripts significantly more stable than those of intronless genes, and this was not related to overa ...[more]
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