Transcription profiling of liver from wistar and kyoto rats exposed to orotic acid to investigate fatty liver disease

ABSTRACT: Although fatty liver disease is caused by a number of toxicological insults and the metabolic syndrome, the exact mechanisms by which many of these pathophysiological stimulii induce fatty liver are unknown. The rapid and profound steatosis caused by orotic acid, resulting from an impairment in the production of ApoB, has been investigated in the Wistar strain rat using a combined transcriptomic and metabonomic/metabolomic approach. Analysis of liver tissue from rats exposed to orotic acid for 1, 3, and 14 days was performed by DNA microarrays and high resolution 1H NMR spectroscopy based metabonomics of both tissue extracts and intact tissue (n = 3). Data were analyzed using a combination of ANOVA and principal components analysis, used as a data reduction tool to visualize the most pertu

ORGANISM(S): Rattus norvegicus

SUBMITTER: Julian Griffin 

PROVIDER: E-BIID-1 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress