Quantification of global transcription patterns in prokaryotes using spotted microarrays

ABSTRACT: We describe an analysis, applicable to any spotted microarray dataset produced using genomic DNA as a reference, which quantifies prokaryotic levels of mRNA on a genome-wide scale. Applying this to Mycobacterium tuberculosis, we validate the technique, show a correlation between level of expression and biological importance, define the complement of invariant genes and analyse absolute levels of expression by functional class to develop ways of understanding an organism's biology without comparison to another growth condition. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-60

ORGANISM(S): Mycobacterium bovis

SUBMITTER: Mike Withers 

PROVIDER: E-BUGS-60 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress