Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

17 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Mycobacterium tuberculosis after treatment with ciprofloxacin to investigate the SOS DNA repair response

ABSTRACT: Data is also available from http://bugs.sgul.ac.uk/E-BUGS-63

INSTRUMENT(S): Variable Atmosphere Incubator, Bioanalyser

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER:

PROVIDER: E-BUGS-63 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin.

O'Sullivan D M DM Hinds J J Butcher P D PD Gillespie S H SH McHugh T D TD

The Journal of antimicrobial chemotherapy 20080916 6

<h4>Objectives</h4>To investigate how the SOS response, an error-prone DNA repair pathway, is expressed following subinhibitory quinolone treatment of Mycobacterium tuberculosis.<h4>Methods</h4>Genome-wide expression profiling followed by quantitative RT (qRT)-PCR was used to study the effect of ciprofloxacin on M. tuberculosis gene expression.<h4>Results</h4>Microarray analysis showed that 16/110 genes involved in DNA protection, repair and recombination were up-regulated. There appeared to be ...[more]

PMID: 18799471

Similar Datasets

Transcription profiling of Mycobacterium tuberculosis infected human dendritic cells and macrophages and M. tuberculosis grown aerobically to investigate host-pathogen cross-talk

Project description:Data is also available from http://bugs.sgul.ac.uk/E-BUGS-58

2008-01-04 | E-BUGS-58 | biostudies-arrayexpress

Transcription profiling of Campylobacter jejuni flagellar mutants to investigate the RpoN and FliA regulons, and the flagellar hook length control gene fliK

Project description:Data is also available from http://bugs.sgul.ac.uk/E-BUGS-50

2007-09-06 | E-BUGS-50 | biostudies-arrayexpress

Responses of Staphylococcus aureus exposed to HCl and organic acid stress.

Project description:Staphylococcus aureus is an important food poisoning bacterium. In food preservation, acidification is a well-known method. Permeant weak organic acids, like lactic and acetic acids, are known to be more effective against bacteria than inorganic strong acids (e.g., HCl). Growth experiments and metabolic and transcriptional analyses were used to determine the responses of a food pathogenic S. aureus strain exposed to lactic acid, acetic acid, and HCl at pH 4.5. Lactic and acetic acid stress induced a slower transcriptional response and large variations in growth patterns compared with the responses induced by HCl. In cultures acidified with lactic acid, the pH of the medium gradually increased to 7.5 during growth, while no such increase was observed for bacteria exposed to acetic acid or HCl. Staphylococcus aureus increased the pH in the medium mainly through accumulation of ammonium and the removal of acid groups, resulting in increased production of diacetyl (2,3-butanedione) and pyrazines. The results showed flexible and versatile responses of S. aureus to different types of acid stress. As measured by growth inhibition, permeant organic acid stress introduced severe stress compared with the stress caused by HCl. Cells exposed to lactic acid showed specific mechanisms of action in addition to sharing many of the mechanisms induced by HCl stress. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-87

2011-04-15 | E-BUGS-87 | biostudies-arrayexpress

Transcription profiling of Staphylococcus aureus treated with tigecycline to determine the effect of the expression of virulence factors in biofilm-associated cells

Project description:Data is also available from <ahref=http://bugs.sgul.ac.uk/E-BUGS-81 target=_blank>BuG@Sbase</a>

2009-11-25 | E-BUGS-81 | biostudies-arrayexpress

Transcription profiling of Haemophilus influenzae cells to characterize dependence of competence-induced transcription on known regulatory factors

Project description:Data is also available from http://bugs.sgul.ac.uk/E-BUGS-20

2007-01-30 | E-BUGS-20 | biostudies-arrayexpress

Transcription profiling of Mycobacterium tuberculosis treated with benzothiazinones which kill M. tuberculosis by blocking arabinan synthesis

Project description:Data is also available from <ahref=http://bugs.sgul.ac.uk/E-BUGS-80 target=_blank>BuG@Sbase</a>

2009-03-19 | E-BUGS-80 | biostudies-arrayexpress

Methionine Sulfoximine Resistance in Mycobacterium tuberculosis Is Due to a Single Nucleotide Deletion Resulting in Increased Expression of the Major Glutamine Synthetase, GlnA1.

Project description:We investigated the effect of methionine sulfoximine (MetSox), a potent inhibitor of glutamine synthetase, on Mycobacterium tuberculosis. M. tuberculosis encodes four glutamine synthetases, of which MetSox targets the type I enzyme encoded by glnA1. Trancriptional profiling revealed that glutamate synthetase (gltB) and a type II glutamine synthetase (glnA3) were induced after exposure to MetSox. In addition, we observed a high rate (10(-5)) of spontaneous resistance to MetSox. All resistant strains had a single-nucleotide deletion in the 5' region of glnA1, and Western analysis revealed that GlnA1 expression was increased in resistant as compared with sensitive strains. These data show that M. tuberculosis can respond to the effect of MetSox inhibition either by up-regulation of GlnA3 or by GlnA1. The high frequency of resistance suggests that MetSox and other compounds specifically targeting GlnA1 are not likely to become successful anti-mycobacterial agents. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-112

2011-09-06 | E-BUGS-112 | biostudies-arrayexpress

Transcription profiling by array to assess epidemic methicillin-resistant Staphylococcus aureus 16 phenotypes induced by PBD conjugates

Project description:Objectives Pyrrolobenzodiazepine (PBD) dimers, tethered through inert propyldioxy or pentyldioxy linkers, possess potent bactericidal activity against a range of Gram-positive bacteria by virtue of their capacity to cross-link duplex DNA in sequence-selective fashion. Here we attempt to improve the antibacterial activity and cytotoxicity profile of PBD-containing conjugates by extension of dimer linkers and replacement of one PBD unit with phenyl-substituted or benzo-fused heterocycles that facilitate non-covalent interactions with duplex DNA. Methods DNase I footprinting was used to identify high-affinity DNA binding sites. A staphylococcal gene microarray was used to assess epidemic methicillin-resistant Staphylococcus aureus 16 phenotypes induced by PBD conjugates. Molecular dynamics simulations were employed to investigate the accommodation of compounds within the DNA helix. Results Increasing the length of the linker in PBD dimers led to a progressive reduction in antibacterial activity, but not in their cytotoxic capacity. Complex patterns of DNA binding were noted for extended PBD dimers. Modelling of DNA strand cross-linking by PBD dimers indicated distortion of the helix. A majority (26 of 43) of PBD-biaryl conjugates possessed potent antibacterial activity with little or no helical distortion and a more favourable cytotoxicity profile. Bactericidal activity of PBD-biaryl conjugates was determined by inability to excise covalently bound drug molecules from bacterial duplex DNA. Conclusions PBD-biaryl conjugates have a superior antibacterial profile compared with PBD dimers such as ELB-21. We have identified six PBD-biaryl conjugates as potential drug development candidates. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-118]

2012-04-17 | E-BUGS-118 | biostudies-arrayexpress

Transcription profiling of Yersinia pseudotuberculosis to determine the importance of the Rcs phosphorelay in its survival and pathogenesis

Project description:Data is also available from http://bugs.sgul.ac.uk/E-BUGS-57

2008-04-04 | E-BUGS-57 | biostudies-arrayexpress

Transcription profiling of Yersinia pseudotuberculosis vagH mutants

Project description:Data is also available from http://bugs.sgul.ac.uk/E-BUGS-48

2007-04-30 | E-BUGS-48 | biostudies-arrayexpress