Transcription profiling of Arabidopsis Col-0 plants leaves 1 and 2
Ontology highlight
ABSTRACT: As part of the Cage consortium, leaves 1 and 2 of Col-0 plants were harvested at differents stages of development as defined by Boyes Scale (1.04, 1.12, and 5.10). The Col-0 was used as a control for the coi1-1 plants as both sets were sown on MS media for 7 days and then transferred to soil (Jiffy pots). These samples were hybridized against the oligo reference labeled with Cy3. These samples can be compared to the Col-WT samples that were harvested by the CAGE members to provide a variation of a normal genomic expression pattern.
Project description:As part of the Cage consortium, all leaves (the shoot) of coi1 plants were harvested at the 1.10 stage of development as defined by Boyes scale. The Col-0 was used as a control for the coi1-1 plants as both sets were sown on MS media for 7 days and then transferred to soil (Jiffy pots). These samples were hybridized against the oligo reference labeled with Cy3. These samples can be compared to the Col-WT samples that were harvested by the CAGE members to provide a variation of a normal genomic expression pattern.
Project description:Expression profiling of the effect of eggs of the Pieris rapae butterfly on Arabidopsis thaliana local leaf vs. distal leaf using 5mm leaf disk
Project description:As part of the Cage consortium, all leaves (the shoot) of Col-4 plants were harvested at the 1.12 stage of development as defined by Boyes scale. These samples were hybridized against the oligo reference labeled with Cy3. These samples can be compared to the Col-WT samples that were harvested by the CAGE members to provide a variation of a normal genomic expression pattern.
Project description:As part of the Cage consortium, leaves 1 and 2 of eds8 plants were harvested at differents stages of development as defined by Boyes scale (1.04, 1.12, and 5.10). These samples were hybridized against the oligo reference labeled with Cy3. These samples can be compared to the Col-WT samples that were harvested by the CAGE members to provide a variation of a normal genomic expression pattern.
Project description:As part of the CAGE consortium, fully opened flowers from Arabidopsis thaliana Col-4 plants at the developmental stage 6.50 (Boyes stage) were hybridized against the oligo reference labeled with Cy3. The flower samples in addition to the leaf, whole shoot, and roots samples from other CAGE members provide a basal/normal genomic expression pattern for the Col-4 wildtype plants.
Project description:As part of the Cage consortium, all leaves (the shoot) of eds8 plants were harvested at the 1.12 stage of development as defined by Boyes scale . These samples were hybridized against the oligo reference labeled with Cy3. These samples can be compared to the Col-WT samples that were harvested by the CAGE members to provide a variation of a normal genomic expression pattern.
Project description:Expression profiling of the affect of eggs of the Pieris brassicae butterfly on Arabidopsis thaliana local leaf vs. the distal leaf using leaf disks the size of the egg batch.
Project description:As part of the CAGE consortium, Col-4 plants (1.04, 1.08, 1.12 or 5.10 stage) were exposed to a methyl jasmonate stimulus (or to the ethanol as a control) and leaves 1 and 2 were harvested. Hybridizations were performed using the oligo reference labeled with Cy3. The stress samples can be analyzed together with the basal/normal genomic expression patterns for Col-4 wildtype plants collectively produced by CAGE members.
Project description:As part of the CAGE consortium, the rosettes of Col-4 samples (1.12 or 5.10 stage) were dried to 60% of their original mass and leaves 1 and 2 were harvested. Control plants were used to extrapolate the mass proportion of leaves 1+2 to the rest of the rosette and those leaves (1&2) were harvested. Hybridizations were performed using the oligo reference labeled with Cy3. The stress samples can be analyzed together with the basal/normal genomic expression patterns for Col-4 wildtype plants collectively produced by CAGE members.
Project description:As part of the CAGE consortium, Col-4 plants (1.04, 1.08, 1.12 or 5.10 stage) were exposed to a methyl jasmonate stimulus (or to the ethanol as a control) and leaves 1 and 2 were harvested. Hybridizations were performed using the oligo reference labeled with Cy3. The stress samples can be analyzed together with the basal/normal genomic expression patterns for Col-4 wildtype plants collectively produced by CAGE members.