Transcription profiling of rat pancreatic beta-cells in response to double stranded RNA and IFNgamma exposure

ABSTRACT: Viral infections and local production of IFNgamma might contribute to beta-cell dysfunction/death in Type 1 diabetes. Double stranded RNA accumulates in the cytosol of viral-infected cells, and exposure of purified rat beta cells to dsRNA (polyinosinic-polycytidylic acid, PIC) in combination with IFNgamma results in beta-cell dysfunction and apoptosis. To elucidate the molecular mechanisms involved in PIC + IFNgamma-effects, we determined the global profile of genes modified by these agents in primary rat beta cells. FACS-purified rat beta cells were cultured for 6 or 24 h in control condition or with IFNgamma, PIC or a combination of both agents. The gene expression profile was analyzed in duplicate with the Affymetrix RG U34A microarray.

ORGANISM(S): Rattus norvegicus

SUBMITTER: John Brestelli 

PROVIDER: E-CBIL-11 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress