Transcription profiling of rat pancreatic beta-cells in response to cytokine exposure and NF-kappa B activation blocking

ABSTRACT: Type 1 diabetes mellitus results from an autoimmune destruction of pancreatic beta-cells. Based on findings suggesting NF-kappa B plays a role in beta cell apoptosis, we blocked NF-kappa B activation in cytokine-exposed FACS sorted beta cells by a recombinant adenovirus (AdI kappa B((SA)2)) containing an inhibitor of NF kappa B alpha (I kappa Bac) super-repressor (S32A/S36A). The expression profile was then analyzed with the Affymetrix RG U34a microarray.

ORGANISM(S): Rattus norvegicus

SUBMITTER: John Brestelli 

PROVIDER: E-CBIL-9 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress