Ontology highlight
ABSTRACT:
The CD34+/CD38- fraction of human umbilical cord blood was parted and either cultivated on AFT024 [kindly provided by I. Lemischka] or without stromal feeder layer for 16h, 20h, 48h or 72h. Both fractions were then harvested and separated by vigorous pipetting and FACS sorted again to separate the HPC from AFT024. Global gene expression profiles were determined using a novel Human Genome cDNA Microarray of over 51,145 ESTs of the UnigenSet-RZPD3. In analogy, we have compared the gene expression profiles of CD34+/CD38- cells versus AFT024 to exclude that differential gene expression resulted from contaminating feeder layer cells.
ORGANISM(S): Mus musculus
SUBMITTER: Wolfgang Wagner
PROVIDER: E-EMBL-3 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Stem cells (Dayton, Ohio) 20050613 8
Cell-cell contact between stem cells and cellular determinants of the microenvironment plays an essential role in controlling cell division. Using human hematopoietic progenitor cells (CD34+/CD38-) and a stroma cell line (AFT024) as a model, we have studied the initial behavioral and molecular sequel of this interaction. Time-lapse microscopy showed that CD34+/CD38- cells actively migrated toward and sought contact with stroma cells and 30% of them adhered firmly to AFT024 stroma through the uro ...[more]