RNAseq vs DeTCT comparison

ABSTRACT: The transcriptome of zebrafish with morphologically mutant phenotypes associated with knockout alleles were analyised using Illumina sequencing to detect differential transcript abundance. More information describing the mutant phenotype can be found at the Wellcome Trust Sanger Institute Zebrafish Mutation Project website http://www.sanger.ac.uk/cgi-bin/Projects/D_rerio/zmp/search.pl?q=zmp_ph7 Total RNA was extracted from morpholically abnormal and sibling wild type embryos from 3 clutches using Trizol. After DNase treatment non-directional RNAseq libraries were made using the TrueSeq protocol with sample indexing. Libraries were 75 base paired-end sequenced by Illumina HiSeq. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Danio rerio

SUBMITTER:  

PROVIDER: E-ERAD-121 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress